a Record control number WOS:000266929700012

a ISSN 1615-9306

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QD31.2

b Item number .Q3 2009

a Personal name Ucakturk, Ebru.

h Medium Electronic Document

a Title Quantitative analysis of ezetimibe in human plasma by gas chromatography-mass spectrometry

c Statement of responsibility, etc Ebru Ucakturk, Nuran Ozaltin, Baris Kaya.

b Name of publisher, distributor, etc Wiley-V C H Verlag Gmbh,

c Date of publication, distribution, etc 2009.

a Summary, etc A new, specific and sensitive GC-MS method with electron impact ionization technique was developed for quantitative analysis of ezetimibe (EZE) in human plasma. Prior to GC analysis, EZE was derivatized with N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA), which is a trimethyl silylating reagent. The derivatization reaction was optimized and parameters such as catalyst, derivatization time, temperature, solvent and the volume of silylating reagent were investigated. Trimethylsilyl ether derivative of EZE was determined in selected ion monitoring (SIM, mass-to-charge ratio (m/z): 326) mode. The method was validated with respect to LOD and LOQ, precision, accuracy, linearity, specificity, stability, and recovery. The LOQ and LOD were found as 15 and 10 ng/mL, respectively. The linearity of the method ranged from 15 to 250 ng/mL. The correlation coefficient of the calibration curve was 0.9977 +/- 0.0004 (+/- S.E.M.). The intra- and inter-day precisions (RSD) were less than 6% and accuracies (bias) for intra- and inter-day accuracy were found between -4.04 and 9.71 % at four different concentration levels (15, 40, 100, 250 ng/mL). The proposed method was successfully applied to real human plasma samples for determination of total EZE.

a Topical term or geographic name as entry element Chemistry

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Ezetimibe

a Topical term or geographic name as entry element GC-MS

a Topical term or geographic name as entry element Human Serum

a Topical term or geographic name as entry element Derivatization

g Relationship information JUN 2009, Vol.38 Issue 11,p1868-1874

t Title Journal Of Separation Science

x International Standard Serial Number 16159306

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1002/jssc.200900078