a Record control number 000278584000004

a ISSN 1572-3887

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QU4.3

b Item number .P87 2010

a Personal name Can, Betul.

a Title Purification and Characterisation of Rat Kidney Glutathione Reductase.

h Medium Electronic Document

c Statement of responsibility, etc Betul Can, Gulnihal Kulaksiz Erkmen, Ozlem Dalmizrak, I. Hamdi Ogus, Nazmi Ozer.

b Name of publisher, distributor, etc Springer,

c Date of publication, distribution, etc 2010.

a Place of publication, distribution, etc New York :

a Summary, etc Glutathione reductase [GR, E.C.1.8.1.7] catalyses NADPH dependent reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Thus, it is the crucial enzyme to maintain high [GSH]/[GSSG] ratio and physiological redox status in cells. Kidney and liver tissues were considered as a rich source of GR. In this study, rat kidney GR was purified and some of its properties were investigated. The enzyme was purified 2,356 fold with a yield of 16% by using heat-denaturation and Sephadex G25 gel filtration, 2',5'-ADP Agarose 4B, PBE94 column chromatographies. The purified enzyme had a specific activity (Vm) of 250 U/mg protein and the ratio of absorbances at wavelengths of A (273)/A (463,) A (280)/A (460), A (365)/A (460), and A (379)/A (463), were 7.1, 6.8, 1.2 and 1.0, respectively. Each mol of GR subunit bound 0.97 mol of FAD. NADH was used as a coenzyme by rat kidney GR but with a lower efficiency (32.7%) than NADPH. Its subunit molecular weight was estimated as 53 kDa. An optimum pH of 6.5 and optimum temperature of 65 A degrees C were found for rat kidney GR. Its activation energy (Ea) and temperature coefficient (Q(10)) were calculated as 7.02 kcal/mol and 1.42, respectively. The Km((NADPH)) and kcat/Km ((NADPH)) values were found to be 15.3 +/- A 1.4 mu M and 1.68 x 10(7) M-1 s(-1) for the concentration range of 10-200 mu M NADPH and when GSSG is the variable substrate, the Km((GSSG)) and the kcat/Km((GSSG)) values of 53.1 +/- A 3.4 mu M and 4.85 x 10(6) M-1 s(-1) were calculated for the concentration range of 20-1,200 mu M GSSG.

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Biochemistry

a Topical term or geographic name as entry element glutathione reductase

a Topical term or geographic name as entry element Rat kidney

g Relationship information MAY 2010, Vol 29 Issue 4, p250-256

x International Standard Serial Number 15723887

a Main entry heading Protein Journal

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1007/s10930-010-9246-4